a-general-method-for-evaluating-incubation-of-sucrose-craving-in-rats

A General Method for Evaluating Incubation of Sucrose Craving in Rats

For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and ...

Aggregation of partially folded intermediates populated during protein folding processes has been described for many proteins. Likewise, partially unfolded chains, generated by perturbation of numerous proteins by heat or chemical denaturants, have also been shown to aggregate readily. However, the process of protein aggregation from native-state conditions is less well understood. Granulocyte-colony stimulating factor (G-CSF), a member of the four-helix bundle class of cytokines, is a therapeutically relevant protein involved in stimulating the growth and maturation of phagocytotic white blood cells. Under native-like conditions (37 degrees C [pH 7.0]), G-CSF shows a significant propensity to aggregate. Our data suggest that under these conditions, native G-CSF exists in equilibrium with an altered conformation, which is highly aggregation prone. This species is enriched in 1-2 M GdmCl, as determined by tryptophan fluorescence and increased aggregation kinetics. In particular, specific changes in Trp58 fluorescence report a local rearrangement in the large loop region between helices A and B. However, circular dichroism, reactivity toward cyanylation, and ANS binding demonstrate that this conformational change is subtle, having no substantial disruption of secondary and tertiary structure, reactivity of the free sulfhydryl at Cys17 or exposure of buried hydrophobic regions. There is no indication that this altered conformation is important to biological activity, making it an attractive target for rational protein stabilization.

Aggregation of granulocyte-colony stimulating factor in vitro involves a conformationally altered monomeric state.

Die Aggregation von Granulozyten-Kolonie-stimulierender Faktor in vitro beinhaltet eine konformativ ver&#xE4;ndert monomeren Zustand.

Konditionierte Signale k&ouml;nnen R&uuml;ckfall zu Drogen- und Lebensmittel-heischendes Verhalten &uuml;ber einen l&auml;ngeren Zeitraum der Abstinenz entlocken. Wenn Suchverhalten mesolimbisches Dopamin-D1-Rezeptoren abh&auml;ngt, sollte die Blockierung dieser Rezeptoren sucht Verhalten reduzieren.

Effekte der systemischen oder Nucleus Accumbens-Regie Dopamin D1-Rezeptor-Antagonismus auf Saccharose in Ratten suchen.

Conditioned cues can elicit relapse to drug- and food-seeking behavior over prolonged periods of abstinence. If seeking behavior depends on mesolimbic dopamine D1 receptors, blocking these receptors should reduce seeking behavior.

Article	Year
Aggregation of granulocyte-colony stimulating factor in vitro involves a conformationally altered monomeric state. Protein science : a publication of the Protein Society\| PubMed ID: 16131655	2005
Effects of systemic or nucleus accumbens-directed dopamine D1 receptor antagonism on sucrose seeking in rats. Psychopharmacology\| PubMed ID: 21318562	2011

Jesse Barnes

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