derivation-of-glial-restricted-precursors-from-e13-mice

Derivation of Glial Restricted Precursors from E13 mice

This is a protocol for derivation of glial restricted precursor (GRP) cells from the spinal cord of E13 mouse fetuses. These cells are early precursors ...

Evaluation of therapy of X-linked adrenoleukodystrophy.

Normally, ventricular cerebrospinal fluid (CSF) contains low levels of all metabolite signals on proton magnetic resonance spectroscopic imaging (MRSI). We present here three cases (two with seizure disorders, one with a central nervous system lymphoma) who presented with unusually elevated CSF signals on MRSI. Based on chemical shifts and in vitro studies (in one case), the signals were assigned to propan-1,2-diol (PD), acetone, and lactate, respectively. These compounds were either exclusively, or more readily, detected in CSF than in brain. Proton MRSI conveniently screens both brain and CSF for abnormal metabolism simultaneously.

Metabolites in ventricular cerebrospinal fluid detected by proton magnetic resonance spectroscopic imaging.

Periventricular leukomalacia, PVL, is the leading cause of cerebral palsy in prematurely born infants, and therefore more effective interventions are required. The objective of this study was to develop an ischemic injury model of PVL in mice and to determine the feasibility of in vivo magnetization transfer (MT) magnetic resonance imaging (MRI) as a potential monitoring tool for the evaluation of disease severity and experimental therapeutics. Neonatal CD-1 mice underwent unilateral carotid artery ligation on postnatal day 5 (P5); at P60, in vivo T2-weighted (T2w) and MT-MRI were performed and correlated with postmortem histopathology. In vivo T2w MRI showed thinning of the right corpus callosum, but no significant changes in hippocampal and hemispheric volumes. Magnetization transfer MRI revealed significant white matter abnormalities in the bilateral corpus callosum and internal capsule. These quantitative MT-MRI changes correlated highly with postmortem findings of reduced myelin basic protein in bilateral white matter tracts. Ventriculomegaly and persistent astrogliosis were observed on the ligated side, along with evidence of axonopathy and fewer oligodendrocytes in the corpus callosum. We present an ischemia-induced mouse model of PVL, which has pathologic abnormalities resembling autopsy reports in infants with PVL. We further validate in vivo MRI techniques as quantitative monitoring tools that highly correlate with postmortem histopathology.

Article	Year
Lancet neurology\| PubMed ID: 21435600	2011
European journal of pediatrics\| PubMed ID: 12029453	2002
The Journal of adolescent health : official publication of the Society for Adolescent Medicine\| PubMed ID: 12401431	2002
Wiener klinische Wochenschrift\| PubMed ID: 12416287	2002
AJNR. American journal of neuroradiology\| PubMed ID: 13679292	2003
Wiener klinische Wochenschrift\| PubMed ID: 14768531	2003
Evaluation of therapy of X-linked adrenoleukodystrophy. Neurochemical research\| PubMed ID: 15139299	2004
Current opinion in neurology\| PubMed ID: 15167059	2004
Metabolites in ventricular cerebrospinal fluid detected by proton magnetic resonance spectroscopic imaging. Journal of magnetic resonance imaging : JMRI\| PubMed ID: 15332258	2004
Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine\| PubMed ID: 15968676	2005
Annals of neurology\| PubMed ID: 16240348	2005
Journal of endodontics\| PubMed ID: 18793926	2008
Journal of oral science\| PubMed ID: 18818477	2008
Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine\| PubMed ID: 19097204	2009
Clinics in perinatology\| PubMed ID: 19944838	2009
Photomedicine and laser surgery\| PubMed ID: 20666572	2010
In vivo magnetization transfer MRI shows dysmyelination in an ischemic mouse model of periventricular leukomalacia. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism\| PubMed ID: 21540870	2011
Journal of magnetic resonance imaging : JMRI\| PubMed ID: 22566357	2012

Ali Fatemi

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