detection-of-viral-rna-by-fluorescence-in-situ-hybridization-fish

Detection of Viral RNA by Fluorescence <em>in situ</em> Hybridization (FISH)

Viruses  that infect cells elicit specific changes to normal cell functions which serve  to divert energy and resources for viral replication. Many ...

The HIV-1 ribonucleoprotein (RNP) contains the major structural protein, pr55(Gag), viral genomic RNA, as well as the host protein, Staufen1. In this report, we show that the nonsense-mediated decay (NMD) factor UPF1 is also a component of the HIV-1 RNP. We investigated the role of UPF1 in HIV-1-expressing cells. Depletion of UPF1 by siRNA resulted in a dramatic reduction in steady-state HIV-1 RNA and pr55(Gag). Pr55(Gag) synthesis, but not the cognate genomic RNA, was efficiently rescued by expression of an siRNA-insensitive UPF1, demonstrating that UPF1 positively influences HIV-1 RNA translatability. Conversely, overexpression of UPF1 led to a dramatic up-regulation of HIV-1 expression at the RNA and protein synthesis levels. The effects of UPF1 on HIV-1 RNA stability were observed in the nucleus and cytoplasm and required ongoing translation. We also demonstrate that the effects exerted by UPF1 on HIV-1 expression were dependent on its ATPase activity, but were separable from its role in NMD and did not require interaction with UPF2.

Article	Year
Unexpected roles for UPF1 in HIV-1 RNA metabolism and translation. RNA (New York, N.Y.)\| PubMed ID: 18369187	2008
Virology\| PubMed ID: 19070880	2009
The Journal of biological chemistry\| PubMed ID: 19737937	2009
Journal of cell science\| PubMed ID: 20053637	2010
Current HIV research\| PubMed ID: 22211663	2011

Lara Ajamian

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