isolation-identification-primary-fibroblast-like-synovial-cells-from

Isolation and Identification of Primary Fibroblast-like Synovial Cells from Patients with Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease characterized by synovial hyperplasia and progressive joint destruction as its ...

To evaluate the clinical efficacy and safety of bushen quhan zhiwang decoction (BQZD) combined methotrexate (MTX) in treating rheumatoid arthritis (RA).

[Treating rheumatoid arthritis patients of Shen deficiency and cold invading syndrome by bushen quhan zhiwang decoction combined methotrexate: an evaluation of clinical efficacy and safety].

Total glucosides of paeony (TGP) is a natural plant extract, which is widely used in China for treating rheumatoid arthritis (RA). Many relevant randomised controlled trials (RCTs) of TGP for RA are available, but they have not been systematically reviewed. This systematic review aims to examine the effectiveness and safety of TGP in patients with RA.

Total glucosides of paeony for rheumatoid arthritis: a protocol for a systematic review.

Total glucosides of paeony (TGP) is commonly used to treat rheumatoid arthritis (RA) in China. However, clinical practice hasn't been well informed by evidence from appropriately conducted systematic reviews. This PRISMA-compliant systematic review aims at examining the effectiveness and safety of TGP for RA.

Total glucosides of paeony for rheumatoid arthritis: A systematic review of randomized controlled trials.

To investigate the clinical characteristics and relevant factors of secondary immune thrombocytopenia (ITP) in patients with primary Sjögren's syndrome (pSS).

Factors associated with secondary immune thrombocytopenia in patients with primary Sjögren's syndrome: a retrospective study of 639 cases.

This study aims to investigate the therapeutic effect of Wangbi Tablets(WBT) on the inflammation in the rat model of collagen-induced arthritis(CIA) with the syndrome of kidney deficiency based on the cyclic guanosine monophosphate-adenosine monophosphate synthase(cGAS)-stimulator of interferon genes(STING) signaling pathway. Eighteen rats were randomly chosen from 24 SPF-grade rats for the modeling of CIA with the syndrome of kidney deficiency. The 24 SPF-grade rats were randomized into 4 groups: control(normal rats), model(CIA with syndrome of kidney deficiency), model+WBT(M+WBT), and model+methotrexate(M+MTX). The syndrome score of kidney deficiency and arthritis index were recorded. The serum levels of interleukin-18(IL-18) and interleukin-1β(IL-1β) were measured by enzyme-linked immunosorbent assay(ELISA). Hematoxylin-eosin staining and safranin O-fast green staining were employed to observe the pathological status of ankle joints, synovium, and cartilage. Immunohistochemistry(IHC) was used to detect the expression of polymerase beta(Polβ) and cGAS in rats. The protein levels of polymerase beta(Polβ), cGAS, STING, phospho-STING(p-STING), nuclear factor-kappa B(NF-κB), phospho-NF-κB(p-NF-κB), interferon regulatory factor 3(IRF3), gasdermin-D(GSDMD), GSDMD N-terminus(GSDMD-NT), and cysteinyl aspartate specific proteinase-1(caspase-1) were determined by Western blot. Terminal-deoxynucleoitidyl transferase-mediated dUTP nick end labeling(Tunel) was employed to examine the apoptosis in the articular joints of rats. The results showed that compared with the control group, the model group showed obvious symptoms and signs of kidney deficiency and arthritis. The ankle joint deformity, mental condition, and coat color were improved by WBT. Compared with the model group, WBT alleviated the symptoms and signs of kidney deficiency and arthritis. Compared with the control group, the modeling elevated the serum levels of IL-18 and IL-1β, which were reduced by WBT, especially the level of IL-18. Compared with the control group, the model group showed a large number of inflammatory cells and damage of the cartilage layer in ankle joint, while WBT alleviated the pathological damage. Compared with the control group, the modeling significantly up-regulated the protein levels of cGAS, IRF3, p-NF-κB/NF-κB, caspase-1, and cleaved-caspase-1 and slightly up-regulated the protein levels of p-STING/STING, GSDMD, and GSDMD-NT. Compared with the model group, WBT significantly up-regulated the protein level of Polβ, significantly down-regulated the protein levels of cGAS, IRF3, GSDMD, and GSDMD-NT, and caspase-1, and slightly down-regulated the protein levels of p-STING/STING, p-NF-κB/NF-κB, and cleaved-caspase-1. Compared with the control group, the model group demonstrated decreased apoptosis in the ankle joint, synovium, and neovascularized endothelium, while WBT mitigated these situations. In conclusion, WBT has a therapeutic effect on CIA rats with the syndrome of kidney deficiency. Specifically, WBT may up-regulate the expression of Polβ in the ankle joint and inhibit the cGAS-STING signaling pathway to down-regulate the expression of executive protein of pyroptosis and reduce the release of cytokines, thus inhibiting inflammation and slowing down the progression of bone destruction.

[Wangbi Tablets reduce inflammation in rat model of collagen-induced arthritis with syndrome of kidney deficiency by regulating cGAS-STING signaling pathway].

Article	Year
[Treating rheumatoid arthritis patients of Shen deficiency and cold invading syndrome by bushen quhan zhiwang decoction combined methotrexate: an evaluation of clinical efficacy and safety]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine\| PubMed ID: 23905378	2013
Total glucosides of paeony for rheumatoid arthritis: a protocol for a systematic review. BMJ open\| PubMed ID: 26962036	2016
Total glucosides of paeony for rheumatoid arthritis: A systematic review of randomized controlled trials. Complementary therapies in medicine\| PubMed ID: 28917375	2017
Factors associated with secondary immune thrombocytopenia in patients with primary Sjögren's syndrome: a retrospective study of 639 cases. Clinical and experimental rheumatology\| PubMed ID: 35383565	2022
[Wangbi Tablets reduce inflammation in rat model of collagen-induced arthritis with syndrome of kidney deficiency by regulating cGAS-STING signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica\| PubMed ID: 39701683	2024

Jian-ming Wang

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