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Genetics
Measuring Gene Expression with RNA-Seq
Description
RNA-seq measures gene expression by sequencing RNA from a sample. It is a high-throughput method, which means it can read many RNA molecules at once. It is especially useful because it can be analyzed without prior genomic information.
In RNA-seq, RNA isolated from samples of interest is first us...
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Transcript
RNA测序（RNA-seq）是一种能够提供细胞群体中所有表达RNA的序列和数量信息的技术，这些RNA统称为“转录组”。与微阵列等其他表达谱分析方法不同，微阵列需要针对已知RNA序列进行探针杂交，而RNA-seq可用于基因组尚未测序的生物体进行基因表达谱分析。此外，与微阵列相比，RNA-seq能够更准确地测量更宽范围的转录本表达水平，尤其是在极低或极高表达水平时。
本视频将介绍RNA-seq的基本原理、RNA-seq文库构建及数据分析的实验方案，以及该技术的一些应用。
首先，让我们回顾一下 RNA-seq 背后的某些原理。转录组测序需要分离出待测定其表达水平的一群...
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