Guest Editors
Guigang Li
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
<p>Dr. Guigang Li is a professor and the chair of the Department of Ophthalmology at Tongji Hospital, part of Tongji Medical College at Huazhong University of Science and Technology. As a specialist in corneal surgery, he has performed over 2,000 keratoplasties and received training at OSREF and the LUSK Eye Center. His research focuses on corneal and ocular surface diseases, eye-banking technologies, and innovations in endothelial transplantation. He is particularly interested in the niche modulation of limbal stem cells and the construction of in vitro organoids. His work has been funded by over 10 national and provincial grants, and he has authored over 50 peer-reviewed publications.</p>
Guanyu Su
Henan Eye Hospital, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University
<p>Dr. Guanyu Su holds both an MD and a PhD. He is an ophthalmologist at Henan Eye Hospital, Henan Provincial People’s Hospital, and the People’s Hospital of Zhengzhou University. He earned a master’s degree from Beijing Tongren Hospital, Capital Medical University, and completed his PhD at Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. His research focuses on limbal stem cell biology and orbital diseases, and he has published five peer-reviewed articles in SCI journals.</p>
Collection Overview
Stem cell isolation and culturing are crucial initial steps that influence the yield, purity, and functional integrity of all downstream applications in regenerative medicine, including disease modeling and cell therapy. This Methods Collection presents rigorously validated protocols for the derivation, expansion, and quality control of embryonic, induced pluripotent, mesenchymal, and tissue-resident stem cells in both human and animal models. It incorporates serum-free media, 3D microenvironments, single-cell cloning, and automated bioreactor workflows. By standardizing variables, identifying common pitfalls, and providing cross-laboratory benchmarking data, this collection enables researchers to produce high-quality cells, minimize batch-to-batch variability, and expedite translational pipelines for drug screening and therapeutic manufacturing.
Articles
Efficient Isolation of Adipose-derived Stem Cells and Adipocytes from Porcine Adipose Tissue
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2026
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Yating Xiao*1, Xinyu Zhou*1, Xuanzhang Tang*1, Nuria García Neira1,2, Ethel Estefania Valenzuela González1,2, Yufei Wang1, Yuan Tang1, Jiaying Yu3, Ziyi Zhao3,4, Mingzhou Li1, Lu Lu1,5,6
1State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, 2Faculty of Pharmacy, Paris Cité University, 3Chengdu DosSense Biotech Co., LTD, 4TCM Prevention and Treatment of Metabolic and Chronic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, 5Department of Pig Production, Chongqing Academy of Animal Science, 6College of Animal Science and Technology, Southwest University