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TOPICAL COLLECTIONS

Cutting-Edge Methodologies for Investigating Plant-Pathogen Interactions
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Guest Editors

Ritu Singh

Ritu Singh

University of California, Davis

<p>Ritu Singh is a plant molecular biologist and geneticist specializing in plant immunity and biotic stress resistance. She earned her PhD from the National Institute of Plant Genome Research (NIPGR), New Delhi, where she focused on genomics-assisted QTL mapping and molecular dissection of biotic stress responses in chickpeas. Currently, Ritu is a postdoctoral scholar at UC Davis, where her research aims to unravel the defense mechanisms of core eudicot plants against generalist pathogens while also investigating the virulence strategies employed by these pathogens. Her enduring passion for understanding the intricate relationships between plants and fungi drives her commitment to advancing knowledge in this critical area of research.&nbsp;</p>

Prem Pratap Singh

Prem Pratap Singh

University of California, Davis

<p>Dr. Prem Pratap Singh, a PhD graduate from Banaras Hindu University, India, is a computational biologist with a deep-rooted passion for deciphering the secrets of natural antimicrobial agents. His journey involves wielding bioinformatics and omics techniques to study plant-derived essential oils and their antimicrobial properties against foodborne pathogens. With a remarkable track record of research publications, he now serves as a postdoctoral scholar at the University of California Davis, delving into the impact of grapevine red blotch virus on grape and wine composition.&nbsp;</p>

Collection Overview

In light of the increasing challenges posed by pathogens in agriculture and their impact on global food security, a Methods Collection dedicated to plant-pathogen interactions is proposed. This collection, a collaborative effort of experts in the field, aims to compile cutting-edge methodologies for studying the intricate molecular, genetic, and biochemical interactions between plants and pathogens. By compiling protocols from these experts, this collection serves as an invaluable resource for researchers to standardize techniques that enhance reproducibility and foster cross-laboratory collaboration. 

Key areas of focus could include: 

  • High-throughput phenotyping approaches for disease resistance 
  • Advanced microscopy and imaging techniques for studying infection processes 
  • Techniques for functional genomics, including CRISPR/Cas9, knockouts, and transcriptome analysis 
  • Methods for studying plant immune responses at the biochemical and molecular levels, such as pathogen effector profiling and immune receptor characterization 

Articles

A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes
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A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes

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2025

Abstracts

Fast-Flowering Mini-Maize as a model pathosystem for studying ear rot diseases under controlled conditions

Harshita Saxena1

1University of Kentucky

Hypoglycemic potential of a lectin from Leucaena leucocephala (Lam.) de Wit seeds demonstrated by in vitro Glucose uptake assay using HepG2 cell line.

Deepti Madayi*1

1Department of Biotechnology, University of Calicut